| Annotated protein: | Neuroligin 4-like (Neuroligin-4) (NL-4). Gene symbol: NLGN4L. Taxonomy: Mus musculus (Mouse). Uniprot ID: B0F2B4 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NLGN4X SynGO data @ NLGN4Y |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | retina, glycinergic |
| Annotated paper: | Hoon M, et al. "Neuroligin-4 is localized to glycinergic postsynapses and regulates inhibition in the retina" Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):3053-8 PMID:21282647 |
| Figure(s): | Fig.1, 5 |
| Annotation description: | Fig.1: "NL4 associates with GlyRs" - " Thus, NL4 is localized to inhibitory, preferentially glycinergic synapses in the retina and is the only NL isoform to display such selectivity." Fig.5: "NL4 interacts with collybistin and gephyrin." "We found that the intracytoplasmic domain of NL4 (NL4ICD) also interacts with gephyrin and collybistin in yeast two-hybrid assays (Fig. 5A) and GST-pulldowns (Fig. 5B). Finally, gephyrin and collybistin are coimmunoprecipitated with NL4 in chemically cross-linked extracts from mixed brainstem and spinal cord samples of WT and NL2-KO, but not of NL4-KO mice (Fig. 5D), indicating that NL4, gephyrin, and collybistin specifically associate into higher-order complexes in vivo." - The direct interaction of the intracellular tail of NLGN4; neuroligin-4 with gephyrin supports localization at the postsynaptic membrane (as do other known neuroligins). - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue Cell-free system |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS Y2H |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2773 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |