| Annotated protein: | Receptor-type tyrosine-protein phosphatase S (R-PTP-S) (EC 3.1.3.48) (PTPNU-3) (Receptor-type tyrosine-protein phosphatase sigma) (R-PTP-sigma). Gene symbol: PTPRS. Taxonomy: Mus musculus (Mouse). Uniprot ID: B0V2N1 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PTPRS |
| Ontology domain: | Biological Process |
| SynGO term: | synapse organization (GO:0050808) |
| Synapse type(s): | Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Horn KE, et al. "Receptor protein tyrosine phosphatase sigma regulates synapse structure, function and plasticity" J Neurochem. 2012 Jul;122(1):147-61 PMID:22519304 |
| Figure(s): | fig 3, 4, 6 |
| Annotation description: | Fig.3: "Increased density of pre-synaptic puncta in cultured cortical neurons from RPTPσ null mice" Fig.4: "Increased spine density and length along proximal primary dendrites of CA1 pyramidal cells in RPTPσ-/- mice" Fig.6: "RPTPσ knockdown does not impact AMPAR mEPSC amplitude but affects AMPAR mEPSC frequency and paired-pulse facilitation" Discussion: "We did, however, detect an increase in the mean frequency of mEPSCs recorded at excitatory synapses, suggesting an increased number of synapses in RPTPσ null mice. This result is consistent with our anatomical findings in vitro and in vivo, demonstrating that neurons in culture exhibit a higher density of VAMP2-positive puncta, and that CA1 hippocampal pyramidal neurons in RPTPσ knockout mice exhibit a higher density of dendritic spines. Our analysis of paired-pulse ratios suggests that Schaffer collateral synapses onto CA1 neurons in RPTPσ null mice have an abnormally low probability of release. In the neocortex, immature synapses typically show an initial high probability of transmitter release that declines as maturation proceeds (Feldmeyer and Radnikow 2009). Therefore, the low probability of release detected in knockouts could correlate with relatively mature pre-synaptic function in RPTPσ null mice. However, increased AMPA mEPSC amplitude is associated with increased post-synaptic maturity (Hsia et al. 1998), yet our mEPSC analysis revealed no differences in the mean amplitude of events recorded in wild-types or RPTPσ null littermates, and therefore does not indicate differences in post-synaptic maturation. Together, these findings support the conclusion that the absence of RPTPσ increases the density of synapses between neurons, but that individual synapses lacking RPTPσ are less effective than wild-type counterparts." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Whole-cell patch clamp |
| Annotator(s): | Hwajin Jung (ORCID:0000-0001-6194-9648) Eunjoon Kim (ORCID:0000-0001-5518-6584) |
| Lab: | Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS) and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea |
| SynGO annotation ID: | 2743 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |