| Annotated protein: | AP-3 complex subunit delta-1 (AP-3 complex subunit delta) (Adaptor-related protein complex 3 subunit delta-1) (Delta-adaptin) (mBLVR1). Gene symbol: AP3D1. Taxonomy: Mus musculus (Mouse). Uniprot ID: O54774 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP3D1 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | striatum hippocampus |
| Annotated paper: | Newell-Litwa K, et al. "Hermansky-Pudlak protein complexes, AP-3 and BLOC-1, differentially regulate presynaptic composition in the striatum and hippocampus" J Neurosci. 2010 Jan 20;30(3):820-31 PMID:20089890 |
| Figure(s): | fig. 1-3 |
| Annotation description: | The authors used an antibody against AP-3 delta for immunofluorescence and immuno EM. The specificity of the antibody was confirmed using an AP-3 delta knockout mouse. From the text "To discern the subcellular compartments where AP-3 is present in neurons, we performed immunofluorescence colocalization of AP-3 in primary cultured neurons. Synaptic elements were identified with antibodies against the synaptic vesicle proteins VAMP2 and synaptophysin in cultured neurons and brain sections, respectively. Deconvolution immunofluorescence microscopy of triple-immunolabeled mouse neocortical primary neurons [embryonic day 18.5 (E18.5); days in vitro (DIV) 14] demonstrated that AP-3, the AP-3 cargo VAMP7-TI, and the synaptic vesicle protein, synaptobrevin/VAMP2, colocalize in discrete punctae along neuronal processes (Fig. 2 A)". "We further defined the ultrastructural and subsynaptic localization of AP-3 by quantitative immunoelectron microscopy. We used the monoclonal antibody against the δ subunit of AP-3 due to the signal specificity using immunoperoxidase detection of antigen-antibody complexes (Fig. 1). AP-3-immunoreactive material was detected at the ultrastructural level with biotinylated anti-mouse antibodies and avidin-biotin peroxidase complex. As suggested by the immunofluorescence microscopy, AP-3 prominently localized to axons and nerve terminals in the striatum as well as in the CA3 region and dentate gyrus of the hippocampus. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 2740 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |