| Annotated protein: | AP-2 complex subunit beta (AP105B) (Adaptor protein complex AP-2 subunit beta) (Adaptor-related protein complex 2 subunit beta) (Beta-2-adaptin) (Beta-adaptin) (Clathrin assembly protein complex 2 beta large chain) (Plasma membrane adaptor HA2/AP2 adaptin beta subunit). Gene symbol: AP2B1. Taxonomy: Homo sapiens (Human). Uniprot ID: P63010 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ AP2B1 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle endocytosis (GO:0048488) |
| Annotated paper: | Morgan JR, et al. "A conserved clathrin assembly motif essential for synaptic vesicle endocytosis" J Neurosci. 2000 Dec 1;20(23):8667-76 PMID:11102472 |
| Figure(s): | fig. 8 and 9 |
| Annotation description: | Please note, this was a rat AP-2 beta2 peptide injected into a squid giant synapse. From the results, "The ability of the AP peptides to inhibit synaptic transmission is consistent with these peptides blocking clathrin-mediated endocytosis in the nerve terminal. We next examined this possibility more directly by examining the ultrastructure of squid presynaptic terminals that were injected with AP2 pep. If AP2 pep inhibits clathrin assemblyin vivo, then this peptide should deplete the nerve terminal of both synaptic vesicles and coated vesicles. Further, the accumulation of vesicular membrane should cause a proportionate increase in the area of the presynaptic plasma membrane (Heuser and Reese, 1973; Morgan et al., 1999). To test these predictions, we injected AP2 pep until synaptic transmission was inhibited by >90%". This was followed by ... electron microscopy. In comparison to controls, terminals injected with AP2 pep were depleted of synaptic vesicles and coated vesicles (Fig.8). We quantified the spatial distribution of synaptic vesicles ...... When normalized to the distribution of vesicles in the control terminals, the AP2 peptide caused a 23% reduction in the docked synaptic vesicles within 50 nm of the active zone and a 66% depletion of vesicles farther away (Fig.9 B). This yielded a 62% overall loss of synaptic vesicles in the AP2 peptide-injected terminals (Fig. 9 C;p < 0.01, Student's t test). We also measured the number of coated vesicles and found that these were depleted by 95%, even more extensively than the synaptic vesicles (Fig. 9 D; p < 0.01, Student's ttest). The two terminals injected with AP2 pep also had a larger plasma membrane area than the control terminals (Fig. 9 E). This increase was attributable to the trapping of synaptic vesicle membrane in the plasma membrane, because the gain in plasma membrane area was very similar to the reduction in synaptic vesicle membrane area (Fig.9 E).... In summary, these results are consistent with AP2 pep blocking synaptic vesicle endocytosis at the clathrin assembly step and provide the final evidence that DLLs are functional clathrin assembly motifs". |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Peter McPherson (ORCID:0000-0001-7806-5662) |
| Lab: | Department of Neurology and Neurosurgery, Montreal Neurological Institute, McGill University, Montreal, QC H3A 2B4, Canada |
| SynGO annotation ID: | 2739 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |