| Annotated protein: | Liprin-alpha-3 (Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3) (PTPRF-interacting protein alpha-3). Gene symbol: PPFIA3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60469 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA3 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle docking (GO:0016081) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Wong MY, et al. "Liprin-alpha3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses" Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2234-2239 PMID:29439199 |
| Figure(s): | Fig 4 |
| Annotation description: | Using a constutitive Liprin a3 knockout (KO), Wong et.al. shows that Liprin a3 mediates synaptic vesicle docking. In transmission electron microscopy of individual sections, KO showed an ~25% reduction in the number of docked vesicles. Tethered vesicles (<100 nm from the active zone) were similarly affected. Similar observations were observed with two different fixation methods (glutaraldehyde and cryo-fixation). The KO is characterized in figure 2, showing absence of the protein in western blot using 3 different antibodies. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 2738 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |