| Annotated protein: | Liprin-alpha-3 (Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3) (PTPRF-interacting protein alpha-3). Gene symbol: PPFIA3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60469 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA3 |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle exocytosis (GO:0016079) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Wong MY, et al. "Liprin-alpha3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses" Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2234-2239 PMID:29439199 |
| Figure(s): | Fig 3 |
| Annotation description: | Using a constutitive Liprin a3 knockout (KO), Wong et.al. shows that Liprin a3 participates in synaptic vesicle exocytosis. In synaptophysin-pHluorin imaging experiments, KO neurons showed a reduction in the total amount of exocytosis. This was rescued by reintroduction of Liprin a3. The KO is characterized in figure 2, showing absence of the protein in western blot using 3 different antibodies. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 2737 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |