| Annotated protein: | Liprin-alpha-3 (Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3) (PTPRF-interacting protein alpha-3). Gene symbol: PPFIA3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P60469 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPFIA3 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic active zone cytoplasmic component (GO:0098831) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Wong MY, et al. "Liprin-alpha3 controls vesicle docking and exocytosis at the active zone of hippocampal synapses" Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2234-2239 PMID:29439199 |
| Figure(s): | Fig 1, 5 |
| Annotation description: | Wong et.al. use STED microscopy to demonstrate that Liprin a3 (gene product of PPFIA3) is localized at the presynaptic active zone. Liprin a3 closely colocalized with the active zone marker Bassoon. (figure 1C,D) Specificity of the antibody for Liprin a3 is demonstrated in Figure 5A, where no signal was detected in liprin a3 knockout cultures. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Super resolution |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| SynGO annotation ID: | 2734 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |