| Annotated protein: | Leucine-rich repeat transmembrane neuronal protein 2 (Leucine-rich repeat neuronal 2 protein). Gene symbol: LRRTM2. Taxonomy: Homo sapiens (Human). Uniprot ID: O43300 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ LRRTM2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic specialization membrane (GO:0099060) |
| Synapse type(s): | cerebral cortex, GABAergic |
| Annotated paper: | Loh KH, et al. "Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts" Cell. 2016 Aug 25;166(5):1295-1307.e21 PMID:27565350 |
| Figure(s): | Fig.1F, S2 |
| Annotation description: | The authors express HRP-fused recombinant LRRTM2 and observe stainging of the synaptic cleft. LRRTM2 is a postsynaptic TM adhesion protein that is known to be able to induce presynaptic differentiation of GLUTergic synapses. The postsynaptic localization is not resolved in these figures (difficult for epitopes/proteins in/around the synaptic cleft) but known from other literature (seen f.i. ticket #545 that shows expression in the postsynapse). This aspect is implicitly acknowledged by the authors since the study uses HPR-tagging of postsynaptic proteins to separate Glut from GABA synapses (Fig.1A) |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2730 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |