| Annotated protein: | Vesicle-fusing ATPase 1 (EC 3.6.4.6) (N-ethylmaleimide-sensitive fusion protein 1) (NEM-sensitive fusion protein 1) (Protein comatose) (Vesicular-fusion protein NSF1) (dNsf-1) (NSF-1). Gene symbol: COMT. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: P46461 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NSF |
| Ontology domain: | Biological Process |
| SynGO term: | synaptic vesicle cycle (GO:0099504) |
| Annotated paper: | Littleton JT, et al. "Temperature-sensitive paralytic mutations demonstrate that synaptic exocytosis requires SNARE complex assembly and disassembly" Neuron. 1998 Aug;21(2):401-13 PMID:9728921 |
| Figure(s): | Fig. 4 |
| Annotation description: | Littleton et al used a Drosophila temperature-sensitive paralytic mutation in NSF (termed "comatose") that slowly blocks synaptic transmission while syntaxin and SNARE complexes accumulate on synaptic vesicles. Figure 4 shows that increased temperature leads to accumulation of 70S SNARE assembled SNARE complex. These findings suggest that NSF mediates disassembly of the SNARE complex after fusion, and that disassembly is required for the re-generation of the SNARE-fusion machinery. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | In this paper, Söllner et al. isolated bovine brain membrane and incubate it with purified NSF and a-SNAP. They found that in the presence of ATP, NSF and a-SNAP the SNARE-complex consisting of synaptobrevin (VAMP-2), syntaxin 1, and SNAP-25 is disassembled in an ATP-dependent manner. @ PMID:8221884 |
| SynGO annotation ID: | 273 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |