| Annotated protein: | Disks large homolog 2 (Channel-associated protein of synapse-110) (Chapsyn-110) (Postsynaptic density protein PSD-93). Gene symbol: DLG2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q91XM9 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLG2 |
| Ontology domain: | Biological Process |
| SynGO term: | structural constituent of postsynaptic density (GO:0098919) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Elias GM, et al. "Synapse-specific and developmentally regulated targeting of AMPA receptors by a family of MAGUK scaffolding proteins" Neuron. 2006 Oct 19;52(2):307-20 PMID:17046693 |
| Figure(s): | Figures 3-8 |
| Annotation description: | Key evidence for "structural constituent of postsynaptic density" from Figures 6-7: "PSD-95 and PSD-93 are jointly required for normal synaptic expression of NMDA-Rs" Knockdown of either PSD-95 or PSD-93 selectively reduced AMPAR transmission. Simultaneous knockdown of both PSD-95 and PSD-93 reduced AMPA-R and NMDA-R EPSCs, suggesting, "PSD-95 and PSD-93 are key structural scaffolding elements of the PSD jointly required for proper synaptic expression of NMDA receptors." Figures 3, 4, 5, 8: PSD-95 and PSD-93 are the dominant PSD-MAGUKs responsible for the synaptic targeting of AMPA receptors at mature synapses. (see annotation for 'neurotransmitter receptor localization to postsynaptic specialization membrane (GO:0099645)' in ticket #2050) Figure 1. shRNAs Against PSD-95, PSD-93, and SAP-102 Cause Selective Knockdown in COS-7 Cells and Hippocampal Neurons. Figure 9. "Slice Culture Scheme" shows how mature vs immature synaptic transmission was assayed in hippocampal slices. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Field recordings Electrophysiology (generic) Western blot Biochemical fractionation (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Figure 3: "Patterns of developmental expression of MAGUKs. P2, P10, P35, and 6 month (6M) rat hippocampus homogenates." Western blot analysis. Figure 4-5: Immunogold labeling of SAP102 and PSD-95 in the CA1 stratum radiatum of the hippocampus during development. Table 2. "Summary of immunogold labeling for PSD-95 and SAP-102" @ PMID:10648730 Fig. 2: PSD-95 and PSD-93 associate with TARPs in brain (coIP). @ PMID:14529722 Fig. 4. "SAP97β overexpression does not enhance synaptic transmission in mature neurons but compensates for PSD-93/-95 double KO." Overexpression of SAP97β in hippocampal slice cultures from WT and PSD-93/-95 double KO mice. SAP97β enhanced AMPAR currents double KO but not WT neurons. NMDAR currents, which are normal in these mice, were unchanged. These data suggest "a virtually complete compensation by SAP97β for the mutant phenotype caused by the elimination of two key PSD-MAGUK proteins." @ PMID:20133708 |
| SynGO annotation ID: | 2728 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |