| Annotated protein: | Vesicle-fusing ATPase 1 (EC 3.6.4.6) (N-ethylmaleimide-sensitive fusion protein 1) (NEM-sensitive fusion protein 1) (Protein comatose) (Vesicular-fusion protein NSF1) (dNsf-1) (NSF-1). Gene symbol: COMT. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: P46461 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NSF |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic cytosol (GO:0099523) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Li T, et al. "The Neurexin/N-Ethylmaleimide-sensitive Factor (NSF) Interaction Regulates Short Term Synaptic Depression" J Biol Chem. 2015 Jul 17;290(29):17656-17667 PMID:25953899 |
| Figure(s): | Fig.5 and Fig.7 |
| Annotation description: | In this paper, it was shown that NSF binds to presynaptic protein Neurexin. The Subcellular Membrane Fractionation showed that NSF has a dynamic distribution between the cytoplasm and plasma membrane (Fig. 5B). For example, depletion of cells from Calcium by ETDA leads to the increase of cytoplasmic NSF (Fig. 5E). Also, it was shown that in Neurexin mutant, the distribution of the NSF is shifted toward the cytoplasmic fraction (Fig. 7 A-D). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Microscopy (generic) Western blot Biochemical fractionation (generic) IP + WB/MSMS Y2H |
| Annotator(s): | Momchil Ninov (ORCID:0000-0002-0808-7003) Mahdokht Kohansalnodehi (ORCID:0000-0002-3898-5197) Reinhard Jahn (ORCID:0000-0003-1542-3498) |
| Lab: | Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, 37077 Göttingen, Germany |
| Additional literature: | In this paper, Weidman, et al showed that NSF can bind the Golgi membrane and incubation with ATP and Mg+2 can release it from membranes and increases the cytosolic fraction. @ PMID:2541136 In this paper, Block et al purified NSF from the supernatant (cytosol) fraction of CHO cells to avoid the necessity of preparing large amounts of Golgi membranes as starting material. NSF is released from Golgi membranes upon incubation with ATP. This experiment documents that a large body of NSF is located in the cytoplasm. @ PMID:3186695 In this paper, it was shown that NSF binds to microtubule-associated protein GABARAP at the postsynapse. This interaction was observed both at cytoplasm and in intracellular membrane compartments (Fig. 8). @ PMID:11461150 |
| SynGO annotation ID: | 272 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |