| Annotated protein: | Mitogen-activated protein kinase 10 (MAP kinase 10) (MAPK 10) (EC 2.7.11.24) (SAPK-beta) (Stress-activated protein kinase JNK3) (c-Jun N-terminal kinase 3) (p54-beta). Gene symbol: MAPK10. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P49187 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MAPK10 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport to postsynaptic membrane (GO:0098969) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yang G, et al. "JNK3 couples the neuronal stress response to inhibition of secretory trafficking" Sci Signal. 2013 Jul 9;6(283):ra57 PMID:23838184 |
| Figure(s): | Figures 2 |
| Annotation description: | Fig.2: "Golgi-associated JNK3 mediates stress-induced reduction of surface AMPAR" - "Given that the palmitoylation of JNK3 in primary neurons was increased after NMDA treatment (Fig. 1F), we investigated whether JNK3 inhibited stress-induced secretory trafficking in neurons by monitoring the trafficking of AMPAR GluR1 in response to NMDA-induced neuronal stress" - "A significant and gradual reduction of surface, but not total, GluR1 was detected in rat hippocampal or cortical neurons challenged with NMDA despite the presence of the JNK inhibitors SP600125 or NIMoE (Fig. 2C and fig. S6, B and C). This reduction of surface GluR1 was mostly prevented by short hairpin RNA (shRNA)-mediated knockdown of endogenous JNK3 (Fig. 2D), which could be reversed by overexpressing the shRNA-resistant kinase-deficient JNK3 mutant (JNK3 KD) but not the kinase-deficient JNK3 KD-CS mutant, which cannot be palmitoylated (Fig. 2E)." 23/2/2018 PIm - The application of NMDA places this data in synaptic context. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Sac1-K2a mutant accumulates in the Golgi; Sac1-LZ (leucine zipper) mutant, is less efficient at shuttling to the Golgi @ PMID:18299350 |
| SynGO annotation ID: | 2719 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |