Annotated protein:Voltage-dependent calcium channel gamma-8 subunit (Neuronal voltage-gated calcium channel gamma-8 subunit) (Transmembrane AMPAR regulatory protein gamma-8) (TARP gamma-8). Gene symbol: CACNG8. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q8VHW5
antibody wiki:
SynGO gene info:SynGO data @ CACNG8
Ontology domain:Cellular Component
SynGO term:integral component of postsynaptic density membrane (GO:0099061)
Synapse type(s):cerebral cortex, glutamatergic
Annotated paper:Tomita S, et al. "Functional studies and distribution define a family of transmembrane AMPA receptor regulatory proteins" J Cell Biol. 2003 May 26;161(4):805-16 PMID:12771129
Figure(s):Figure 6A
Annotation description:TARP γ-8 is an integral membrane protein found in the postsynaptic density membrane.

Fig. 6A: "TARPs colocalize with AMPARs at excitatory synapses." Subcellular fractionation and western blot analysis showed TARP γ-8 is enriched in synaptosomes and is resistant to extraction from synaptic membranes with Triton X-100 (PSDI-II fractions), indicating a strong association with the PSD.
'integral component of postsynaptic density membrane (GO:0099061)' is based on (1) the transmembrane topology of the TARP γ-8 and (2) the known interaction with AMPARs (plasma membrane proteins) at the PSD
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Western blot
Biochemical fractionation (generic)
Annotator(s):Hana Goldschmidt (ORCID:0000-0002-5676-366X)
Richard Huganir (ORCID:0000-0001-9783-5183)
Lab:Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA
SynGO annotation ID:2718
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology