| Annotated protein: | Calcineurin subunit B type 1 (Protein phosphatase 2B regulatory subunit 1) (Protein phosphatase 3 regulatory subunit B alpha isoform 1). Gene symbol: PPP3R1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q63810 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP3R1 |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | forebrain, glutamatergic |
| Annotated paper: | Biesemann C, et al. "Proteomic screening of glutamatergic mouse brain synaptosomes isolated by fluorescence activated sorting" EMBO J. 2014 Jan 13;33(2):157-70 PMID:24413018 |
| Figure(s): | Supplemental Table 2 |
| Annotation description: | The authors used synaptosomes derived from vGlut-Venus knockin mice to enrich for glutamatergic presynaptic terminals. Mass spectrometry was performed to detect protein content, which identified PPP3R1. Although this method favors the detection of presynaptic proteins, postsynaptic or extrasynaptic localization of PPP3R1 cannot be excluded with this method. 22/11/2017 Pim - Protein listed under accession gi|84794597 in Table S2. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | none of the above |
| Evidence tracking, Experiment Assay: | Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | Wilhelm et.al. deployed a similar technique based on a synaptosomal preparation and mass spectrometry. This study also detected PPP3R1. @ PMID:24876496 |
| SynGO annotation ID: | 271 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |