| Annotated protein: | Voltage-dependent calcium channel gamma-7 subunit (Neuronal voltage-gated calcium channel gamma-7 subunit) (Transmembrane AMPAR regulatory protein gamma-7) (TARP gamma-7). Gene symbol: CACNG7. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62957 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CACNG7 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | cerebellum, glutamatergic |
| Annotated paper: | Kato AS, et al. "New transmembrane AMPA receptor regulatory protein isoform, gamma-7, differentially regulates AMPA receptors" J Neurosci. 2007 May 2;27(18):4969-77 PMID:17475805 |
| Figure(s): | Figures 2, 8; table 1 |
| Annotation description: | TARP γ-7 is an integral membrane protein belonging to the type II family of TARPs. Fig. 2: "AMPA receptor subunits are the major binding partners for γ-7." coIP-MS analysis identified GluA1-4 in TARP γ-7-immunoprecipitates prepared from rat cerebellar membranes (Table 1). CoIP and western blot analysis of cerebellar extracts confirmed the interaction of γ-7 with GluA1, and to a lesser extent GluA2/3 coimmunoprecipitated with γ-7 (Fig. 2C). Little interaction was detected among the TARP isoforms (Fig. 2C). [Fig. 7E: "High-power magnification shows γ-7 immunoreactivity in the somatodendritic regions of Purkinje cells and in glomeruli of the granule cell layer." Immunostaining of sagittal brain sections with antibodies to γ-7, γ-2/3/4/8, GluA1 and GluA2.] Fig. 8: "γ-7 is enriched in cerebellar postsynaptic densities and binds to PSD-95." Subcellular fractions from rat cerebellum were assayed by Western blotting. γ-7 is more insoluble than γ-5 in cerebellar membrane fractions, "supporting the differential subcellular localization of γ-7 and γ-5." γ-7 immunoprecipitates PSD-95 from cerebellar brain extracts (Fig. 8C). Fig. 1: Antibody specificity for TARP γ-7. Western blot analysis of transfected HEK cells (TARP cDNAs) and adult rat brain extracts from olfactory bulb, cerebral cortex, striatum, hippocampus, Thalamus, cerebellum, brainstem. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) IP + WB/MSMS Protein-protein interaction (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | PMID:12771129 |
| SynGO annotation ID: | 2708 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |