Annotated protein:Regulator of G-protein signaling 7-binding protein (R7 family-binding protein). Gene symbol: RGS7BP. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8BQP9
antibody wiki:
SynGO gene info:SynGO data @ RGS7BP
Ontology domain:Cellular Component
SynGO term:anchored component of postsynaptic density membrane (GO:0099031)
Synapse type(s):striatum, glutamatergic
Annotated paper:Anderson GR, et al. "Expression and localization of RGS9-2/G 5/R7BP complex in vivo is set by dynamic control of its constitutive degradation by cellular cysteine proteases" J Neurosci. 2007 Dec 19;27(51):14117-27 PMID:18094251
Figure(s):Fig. 2, 3
Annotation description:Results section: "RGS9-2 colocalizes with R7BP at postsynaptic sites of striatal neurons"

-Literal: "Although the postembedding procedure is less sensitive and does not provide perfect ultrastructural preservation, it is the only suitable method for unequivocal localization of proteins at synaptic sites (for detailed discussion, see Lujan, 2004). Data presented in Figure 2 establishes that strong immunoreactivity for both R7BP and RGS9-2 is observed at postsynaptic densities of excitatory synapses in which they are found to colocalize."

Fig.3: "RGS9-2 and R7BP share the same subcellular localization in striatum"
- " A-C, Immunoparticles for RGS9-2 were detected associated or close (arrows) to the extrasynaptic plasma membrane of dendritic shafts (Den) and dendritic spines (s) establishing excitatory asynapses with axon terminals (b). Some immunoparticles for RGS9-2 were also detected intracellularly (crossed arrows) associated with intracellular membranes of dendritic shafts and spines. To a lesser extent, RGS9-2 immunoparticles were detected at presynaptic sites associated with the plasma membrane of axon terminals (b) (arrowheads)."

R7BP; Rgs7bp is a palmitoylated protein with a known membrane association via this lipid anchor: [Uniprot] "When palmitoylated, it targets the R7-Gbeta5 complexes to the plasma membrane, leading to inhibit G protein alpha subunits. When it is unpalmitoylated, the R7-Gbeta5 complexes undergo a nuclear/cypolasmic shuttling."
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Antibody (detection)
Evidence tracking, Experiment Assay:Electron Microscopy
Annotator(s):Rita Reig-Viader (ORCID:0000-0002-6893-6177)
Àlex Bayés (ORCID:0000-0002-5265-6306)
Lab:Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra
SynGO annotation ID:2703
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology