| Annotated protein: | Vacuolar protein sorting-associated protein 35 (Maternal-embryonic 3) (Vesicle protein sorting 35). Gene symbol: VPS35. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | brain, glutamatergic cerebral cortex, glutamatergic |
| Annotated paper: | Tian Y, et al. "VPS35-deficiency results in an impaired AMPA receptor trafficking and decreased dendritic spine maturation" Mol Brain. 2015 Oct 31;8(1):70 PMID:26521016 |
| Figure(s): | Fig S2, 4 |
| Annotation description: | Fig.S2: "VPS35 interaction with GluA1 and GluA2" - "To understand how VPS35 regulates AMPAR trafficking, we asked whether AMPARs (GluA1 and GluA2) interact with VPS35 and are cargos of retromer. Indeed, both GFP-GluA1 and GFP-GluA2 were co-localized with endogenous VPS35 in hippocampal neurons, particularly in the shaft of dendrites (Additional file 2: Figure S2A-S2B). GFP-GluA1 was also detected in the Flag-tagged VPS35 immunoprecipitated complex in HEK293 cells (Additional file 2: Figure S2C). These results support the view for AMPAR (e.g., GluA1) as a cargo of VPS35/retromer." Fig.4: VPS35 is present in synaptosomes and in PSD preparations. The authors remark that VPS35 is concentrated in shaft of dendrites, this supports the postsynaptic localization suggested by fractionation experiments. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons Non-neuronal tissue |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| Additional literature: | See also Fig.1 @ PMID:25416282 |
| SynGO annotation ID: | 2701 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |