Annotated protein:Beta-adducin (Add97) (Erythrocyte adducin subunit beta). Gene symbol: ADD2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9QYB8
antibody wiki:
SynGO gene info:SynGO data @ ADD2
Ontology domain:Biological Process
SynGO term:synapse assembly (GO:0007416)
Annotated paper:Bednarek E, et al. "beta-Adducin is required for stable assembly of new synapses and improved memory upon environmental enrichment" Neuron. 2011 Mar 24;69(6):1132-46 PMID:21435558
Figure(s):Fig.2, 3, 4, 5, 6
Annotation description:Summary: Enriched environment triggers structural synaptic plasticity in the hippocampus that requires beta-adducin.

Fig.2: "Enhanced Synapse Turnover in Mature Organotypic Slice Cultures Lacking β-Adducin"
- "By contrast, β-Adducin-/- LMTs exhibited dramatic remodeling of filopodia and satellites (Figures 2A and 2B), suggesting enhanced synapse turnover in the absence of β-Adducin....reintroduction of GFP-β-Adducin into granule cells restored the stability of satellites and filopodia at LMTs of transduced neurons (Figure 2B)."
- "we monitored spine turnover in the stratum radiatum of hippocampal CA1 in mature wild-type and β-Adducin-/- slice cultures. In the absence of β-Adducin, spine gains and losses were more than twice as frequent as in wild-type cultures (Figures 2C and 2D)" ..."we reintroduced the protein into CA1. The GFP-β-Adducin construct ...completely restored spine stability (Figure 2D)."

Fig.3: "Specific Increase in AZ Lability, but No Alterations in Synapse Numbers in β-Adducin-/- Mice Housed under Control Conditions"

Fig.4: "Synapse Disassembly in Enriched Mice Depends on phosphorylation of β-Adducin Involving PKC"

Fig.5: "Failure to Increase Synapse, but Not Thorn Numbers at LMTs upon Enrichment in Mice Lacking β-Adducin"

Fig.6: "Failure to Increase Synapse, but Not Spine Numbers in CA1 upon Enrichment in Mice Lacking β-Adducin"

Model: Fig.8 "Relationship between Learning, Environmental Enrichment, and Connectivity Rearrangements in the Adult"

Discussion: "We have shown that mice lacking β-Adducin have a specific deficit to assemble new synapses under conditions of enhanced plasticity"

Note: I did not select the "regulation of..." version of the 'synapse assembly (GO:0007416)' term because of the known role of beta-adducin as a F-actin capping protein.
Evidence tracking, Biological System:Intact tissue
Evidence tracking, Protein Targeting:Genetic transformation (eg; knockout, knockin, mutations)
Over-expression
Evidence tracking, Experiment Assay:Confocal
Western blot
Annotator(s):Pim van Nierop (ORCID:0000-0003-0593-3443)
Guus Smit (ORCID:0000-0002-2286-1587)
Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
Additional literature:See also @ PMID:27480796

See also @ PMID:23106536
SynGO annotation ID:2687
Dataset release (version):20231201
View annotation as GO-CAM model:Gene Ontology