| Annotated protein: | Tyrosine-protein kinase (EC 2.7.10.2). Gene symbol: ABL1. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: E9PT20 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABL1 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Perez de Arce K, et al. "Synaptic clustering of PSD-95 is regulated by c-Abl through tyrosine phosphorylation" J Neurosci. 2010 Mar 10;30(10):3728-38 PMID:20220006 |
| Figure(s): | Fig.1, 4 |
| Annotation description: | Fig.1: "c-Abl is present in synaptic sites in cultured hippocampal neurons" - "To test whether c-Abl is present at synaptic sites in cultured neurons, we examined the colocalization with the presynaptic marker Syn I. As observed in Figure 1B, at 14 DIV, c-Abl clusters are apposed closely to Syn I puncta, suggesting that this kinase is mainly distributed postsynaptically (Fig. 1B) as reported previously in hippocampal Schaffer collateral-CA1 synapse (Moresco et al., 2003)...Along the dendritic shaft, we observed that GFP-c-Abl-WT clusters colocalize with PSD-95 puncta in close apposition to Syn I (Fig. 1C). The Mander's overlap coefficient (R) shows that c-Abl distribution presents a higher overlap degree with PSD-95 rather than Syn I (RPSD-95 = 0.6343 ± 0.01 vs RSyn I = 0.3517 ± 0.02). These results strongly suggest that c-Abl is mainly localized on postsynaptic structures." Fig.4: "c-Abl interacts with PSD-95" - "The immunoprecipitation with anti-c-Abl antibody followed by PSD-95 immunoblot showed that this postsynaptic scaffold protein forms part of a complex with c-Abl and NR1 (Fig. 4A)" - "c-Abl is enriched in synaptosomal and PSD fractions from adult rat brain (Fig. 4C), validating the postsynaptic distribution of c-Abl in vivo" - "In total brain homogenate and synaptosomal fraction 1, c-Abl was coimmunoprecipitated with PSD-95 and vice versa (Fig. 4D)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2676 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |