| Annotated protein: | Tyrosine-protein kinase ABL2 (EC 2.7.10.2) (Abelson murine leukemia viral oncogene homolog 2) (Abelson tyrosine-protein kinase 2) (Abelson-related gene protein) (Tyrosine-protein kinase ARG). Gene symbol: ABL2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q4JIM5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABL2 |
| Ontology domain: | Biological Process |
| SynGO term: | synapse maturation (GO:0060074) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Xiao X, et al. "Disruption of Coordinated Presynaptic and Postsynaptic Maturation Underlies the Defects in Hippocampal Synapse Stability and Plasticity in Abl2/Arg-Deficient Mice" J Neurosci. 2016 Jun 22;36(25):6778-91 PMID:27335408 |
| Figure(s): | Fig.1, 2, 3, 4, 7, 8, 9 |
| Annotation description: | Background (from abstract): "Immature glutamatergic synapses in cultured neurons contain high-release probability (Pr) presynaptic sites coupled to postsynaptic sites bearing GluN2B-containing NMDA receptors (NMDARs), which mature into low-Pr, GluN2B-deficient synapses ... Loss of the integrin-regulated Abl2/Arg kinase in vivo yields a subpopulation of "immature" high-Pr, GluN2B+ hippocampal synapses ... High-Pr, GluN2B+ synapses are evident in arg-/- animals as early as postnatal day 21 (P21), a time that precedes any observable defects in synapse or dendritic spine number or structure in arg-/- mice...We also demonstrate that, although LTP and LTD are normal in P21 arg-/- slices, both forms of plasticity are significantly altered by P42. These data demonstrate that the integrin-regulated Arg kinase coordinates the maturation of presynaptic and postsynaptic compartments in a subset of hippocampal synapses in vivo, and this coordination is critical for NMDAR-dependent long-term synaptic stability and plasticity." Fig.1: "Pr is increased at synapses in arg-/- hippocampal slices" Fig.2: "Arg-deficient slices exhibit unaffected AMPAR function and larger NMDAR-mediated currents" Fig.3: "arg-/- CA1 neurons have enhanced synaptic NMDAR function" Fig.4: "arg-/- CA1 neurons have longer NMDAR-EPSC decay time constants" Fig.7: "Loss of Arg increases GluN2B-mediated glutamate uncaging-induced NMDAR-EPSCs in a subpopulation of dendritic spines" Fig.8: "Spine head area correlates with NMDAR-uEPSC amplitude only in arg-/- CA1 neurons" Fig.9: "arg-/- slices exhibit age-dependent alterations in NMDAR-dependent LTP and LTD" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Two-photon microscopy Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2674 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |