| Annotated protein: | VPS10 domain-containing receptor SorCS3. Gene symbol: SORCS3. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q8VI51 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ SORCS3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Breiderhoff T, et al. "Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction" PLoS One. 2013 Sep 19;8(9):e75006 PMID:24069373 |
| Figure(s): | Fig.1 |
| Annotation description: | Figure 1: "SORCS3 localizes to the post-synaptic density of hippocampal neurons" - "SORCS3 was detected in fractions encompassing the synaptic plasma membrane or the postsynaptic density (PSD), but not in synaptic vesicle-enriched fractions. The localization of SORCS3 at the PSD was further supported by unbiased proteomics approaches to identify proteins that interact with the cytoplasmic tail of the receptor in neurons...Two prominent proteins were purified on CS3-CT columns that were identified as PSD93 and PSD95 by mass spectrometry (Figure 1B). These proteins were not recovered on columns containing a carboxyl terminal peptide of the related receptor SORCS2 (CS2-CT, Figure 2B). The ability of SORCS3 to interact with PSD95 was confirmed in transiently transfected COS7 cells showing co-immunoprecipitation of SORCS3 with an antiserum directed against PSD95 (Figure 1C)." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2665 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |