| Annotated protein: | Vacuolar protein sorting-associated protein 35. Gene symbol: VPS35. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: Q7KVL7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynaptic endocytic zone (GO:0098833) |
| Synapse type(s): | Neuro-muscular junction |
| Annotated paper: | Inoshita T, et al. "Vps35 in cooperation with LRRK2 regulates synaptic vesicle endocytosis through the endosomal pathway in Drosophila" Hum Mol Genet. 2017 Aug 1;26(15):2933-2948 PMID:28482024 |
| Figure(s): | fig 2a,b,d, s1, s2e, 5a-e |
| Annotation description: | From Results section: "specific but reduced vesicular signals of dVps35 were still detected at the boutons of dVps35-deficient larvae (Fig. 2A and B, Supplementary Material, Fig. S2E)" (...) "the distribution of dVps35-positive vesicular signals in synapse membrane regions decreased in dVps35-deficient larvae. The introduction of dVps35 WT and DN fully rescued the shrunk bouton phenotype, whereas only dVps35 WT facilitated the perimembranous localization of dVps35-positive vesicles (Fig. 2A and D). Approximately, half of dVps35 vesicles localized around the synapse membrane in dVps35+/+ animals, and some of them bordered the synaptic release region active zones (AZs) (white arrowheads in Fig. 2A)." "dVps35 immunosignals were distributed at the edges of active zones (Fig. 5A and B), inside the boutons (Fig. 5C) and in the SSR regions (Fig. 5D and E)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2664 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |