| Annotated protein: | Vacuolar protein sorting-associated protein 35. Gene symbol: VPS35. Taxonomy: Drosophila melanogaster (Fruit fly). Uniprot ID: Q7KVL7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of synaptic vesicle endocytosis (GO:1900242) |
| Synapse type(s): | dopaminergic Neuro-muscular junction |
| Annotated paper: | Inoshita T, et al. "Vps35 in cooperation with LRRK2 regulates synaptic vesicle endocytosis through the endosomal pathway in Drosophila" Hum Mol Genet. 2017 Aug 1;26(15):2933-2948 PMID:28482024 |
| Figure(s): | fig 3, s3 and 4a,c-e |
| Annotation description: | From Results section: "Ultrastructural analysis of dVps35-deficient boutons revealed that SVs decreased in number and increased in size (Fig. 3A and B, Supplementary Material, Fig. S3A). The appearance of cisternal structures also increased in the periactive zones (Fig. 3A and B), which was likely to derive from larger endocytic vesicles (Fig. 3C and D)." (...) "The complementary expression of dVps35 WT but not DN in dVps35-deficient flies almost rescued these SV phenotypes (Fig. 3A and B)." "The loss of dVps35 impaired spontaneous endocytosis at synapse boutons and reduced the amount of SV reserve pools, phenotypes that were rescued by the introduction of dVps35 WT but not DN (Fig. 3E and F, Supplementary Material, Fig. S3B)." (...) "dVps35 heterozygous mutation and DA neuron-specific dVps35 knockdown both exhibit defects in SV release (Supplementary Material, Fig. S3C), which were again rescued by the expression of dVps35 WT but not DN (Supplementary Material, Fig. S3C)." "...the miniature excitatory junction potential (mEJP) amplitude was increased by loss of dVps35 (Fig. 4A)" (...) "The quantal content, which represents the amount of neurotransmitter released from presynapses, was decreased in dVps35 animals (Fig. 4C)." "Spike amplitudes were mildly decreased during 10-min stimulations in dVps35+/+ larvae, which indicated that SV regeneration was achieved effectively. Loss of a copy of dVps35 caused a larger reduction ratio. The introduction of dVps35 WT at the endogenous level rescued this defect, whereas the introduction of dVps35 DN under the same condition appeared less stable in proteins and did not rescue the EJP reduction of dVps35+/- flies" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal Fluorescence recovery after photobleaching (FRAP) Whole-cell patch clamp |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2663 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |