| Annotated protein: | Glutamate receptor ionotropic, NMDA 3A (GluN3A) (N-methyl-D-aspartate receptor) (N-methyl-D-aspartate receptor subtype 3A) (NMDAR3A) (NR3A) (NMDAR-L). Gene symbol: GRIN3A. Taxonomy: Mus musculus (Mouse). Uniprot ID: A2AIR5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIN3A |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic modulation of chemical synaptic transmission (GO:0099171) |
| Synapse type(s): | neocortex, glutamatergic |
| Annotated paper: | Larsen RS, et al. "Synapse-specific control of experience-dependent plasticity by presynaptic NMDA receptors" Neuron. 2014 Aug 20;83(4):879-93 PMID:25144876 |
| Figure(s): | Fig.2, 3, 5 |
| Annotation description: | Background: "Two forms of tLTD are expressed at L2/3 synapses within developing sensory cortices: one that depends on calcium influx through postsynaptic NMDARs (Froemke et al., 2005), and another that depends on preNMDAR-mediated signaling (Banerjee et al., 2009; Bender et al., 2006; Corlew et al., 2007; Rodríguez-Moreno et al., 2011)." Fig.2: "L4-L2/3 tLTD in Dark-Reared Mice Requires PreNMDARs and Is Lost after Visual Experience" - "These data suggest that tLTD in DR mice requires glutamate binding and ionotropic signaling through nonpostsynaptic, and putatively presynaptic, NMDARs, similar to what is observed during early visual cortical development (Corlew et al., 2007)." Fig.3: "Dark Rearing Enhances PreNMDAR-Mediated Glutamate Release at High Frequencies" - "... these data indicate that visual experience alters presynaptic glutamate release at L2/3 synapses in a frequency- and NMDAR-dependent manner." Fig.5: "Dark Rearing Upregulates the GluN3A Subunit, which Is Required for tLTD Induction in DR Mice" - "Dark rearing also increased the expression of the GluN3A NMDAR subunit compared to age-matched NR controls (Figure 5B). This suggests a previously unknown regulation of synaptic GluN3A expression by visual experience." - "dark rearing preserved tLTD induction in wild-type, but not Grin3A-/-, mice (Figures 5D and 5E). " - "These results demonstrate that visual deprivation-induced enhancement of tLTD and glutamate release requires GluN3A" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2656 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |