| Annotated protein: | Vacuolar protein sorting-associated protein 35 (Maternal-embryonic 3) (Vesicle protein sorting 35). Gene symbol: VPS35. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Biological Process |
| SynGO term: | neurotransmitter receptor transport, endosome to postsynaptic membrane (GO:0098887) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Temkin P, et al. "The Retromer Supports AMPA Receptor Trafficking During LTP" Neuron. 2017 Apr 5;94(1):74-82.e5 PMID:28384478 |
| Figure(s): | fig 3c, d, e |
| Annotation description: | From results section: "To track the membrane delivery of SEP-GluA1, which is fluorescent at neutral pH, but not in the acidic environment of endosomes (Miesenböck et al., 1998), we photobleached the entire cell and then induced chemical LTP (Ahmad et al., 2012; Jurado et al., 2013). In control cells, surface SEP-GluA1 increased following LTP induction, while this increase was absent in VPS35 KD cells and in uninduced control cells (Figures 3C)." (...) "To assess whether trafficking was generally perturbed in VPS35-KD cells, we assayed trafficking of the transferrin receptor (TFRC), a nutrient uptake receptor that constitutively traverses multiple pathways in recycling to the plasma membrane from endosomes (Maxfield and McGraw, 2004). TFRC-SEP recovery after photobleaching was unaltered in VPS35-KD neurons (Figure 3D)." "To more directly assess possible effects of VPS35 KD on AMPAR endosomal trafficking, we performed antibody feeding experiments to label surface-GluA1-containing AMPARs. (...) VPS35 KD shifted the distribution of internalized AMPARs away from the early endosomal compartment as defined by colocalization with EEA1 (Figure 3E)." |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Fluorescence recovery after photobleaching (FRAP) |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| Additional literature: | from deleted ticket #2627 human VPS35 fig 5, 6, 7e-f Description: From Results section: "After 7 days' expression (div17), we quantified GluR1 surface turnover by Fluorescence recovery after photobleaching (FRAP) of SEP-GluR1 positive puncta (Fig. 5). SEP-GluR1 clusters were identified on dendrites and photobleached as a small region of interest (ROI) (Fig. 5A). We found overexpression of VPS35 WT resulted in a significant decrease in SEP-GluR1 recovery levels relative to p.D620N or control at spines (Fig. 5B and C). " "To test this, we assayed synaptic activity and GluR1-containing AMPAR function by whole-cell patch-clamp recording and analysis of miniature excitatory postsynaptic currents (mEPSCs) in div21-26 cortical neurons expressing VPS35 WT or p.D620N. (...) Thus, synaptic transmission and/or connectivity are reduced by VPS35 overexpression and potentially regulated by VPS35 activity. The amplitude of mEPSCs, determined by postsynaptic AMPAR number, was significantly decreased in neurons expressing VPS35 WT relative to control and p.D620N (both GFP- and V5-tagged VPS35; Fig. 6A and C and Supplementary Material, Fig. S4)." "by examining the intensity of the endogenous GluR1 clusters in these cells we found a significant increase in GluR1 cluster intensity in the p.D620N expressing human dopaminergic neurons (Fig. 7E and F)." 22/2/2018 Pim - Authors conclude "This impaired fluorescence recovery indicates that VPS35 WT overexpression results in either (i) an increase in immobile GluR1 on the membrane surface or (ii) a reduction in the rate of GluR1 delivery to the surface (26)" @ PMID:25416282 |
| SynGO annotation ID: | 2651 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |