| Annotated protein: | Alpha/beta hydrolase domain-containing protein 17B (Abhydrolase domain-containing protein 17B) (EC 3.1.2.22). Gene symbol: ABHD17B. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q6AY17 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ ABHD17B |
| Ontology domain: | Biological Process |
| SynGO term: | metabolism |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Yokoi N, et al. "Identification of PSD-95 Depalmitoylating Enzymes" J Neurosci. 2016 Jun 15;36(24):6431-44 PMID:27307232 |
| Figure(s): | Fig.5, 6, 8 |
| Annotation description: | Fig.5: "Expression of ABHD17 selectively depalmitoylates PSD-95 in neurons" - "When wild-type ABHD17B was overexpressed by an AAV vector, the amount of di-palmitoylated PSD-95 was greatly reduced and that of non-palmitoylated PSD-95 was inversely increased (Fig. 5). " Fig.6: "Expression of ABHD17 reduces synaptic clustering of PSD-95 and AMPA receptor in neurons" - "Consistent with biochemical data, expression of ABHD17B dramatically reduced the intensity of synaptic PSD-95 clusters (Fig. 6A,B) ... Because palmitoylation-deficient GluA1 mutant shows the normal cell-surface distribution (Hayashi et al., 2005), it is suggested that the reduction of synaptic GluA1 is mediated by decreased PSD-95 scaffolds rather than slight GluA1 depalmitoylation." Fig.8; "ABHD17 specifically mediates PSD-95 depalmitoylation in neurons. " - "When we simultaneously knocked-down ABHD17A, 17B, and 17C by the AAV-miRNA vector (Fig. 3E), ... Importantly, triple-knockdown of ABHD17 members apparently delayed PSD-95 depalmitoylation process (Fig. 8A, B) and significantly prolonged the half-life of palmitate on PSD-95 to 4.8 ± 1.3 h (Fig. 8D). When neurons were treated with 2-BP for 2 h, the intensity of synaptic PSD-95 clusters was significantly reduced (Fig. 8E,F), indicating rapid depalmitoylation of PSD-95 at local synapses. Consistent with biochemical data, this reduction of PSD-95 clusters was significantly suppressed in neurons in which ABHD17 members were knocked-down. " |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2631 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |