| Annotated protein: | Vacuolar protein sorting-associated protein 35 (Maternal-embryonic 3) (Vesicle protein sorting 35). Gene symbol: VPS35. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9EQH3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ VPS35 |
| Ontology domain: | Cellular Component |
| SynGO term: | presynapse (GO:0098793) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Vazquez-Sanchez S, et al. "VPS35 depletion does not impair presynaptic structure and function" Sci Rep. 2018 Feb 14;8(1):2996 PMID:29445238 |
| Figure(s): | fig 1 |
| Annotation description: | From Results section: "Approximately 22% of VPS35 immunoreactivity showed also VAMP2/synaptobrevin reactivity (Manders' coefficient M1: 0.22 ± 0.01), while approximately 35% of synapses contained VPS35 signal (Manders' coefficient M2: 0.35 ± 0.02) (Fig. 1a,b). " (...) "VPS35 immunoreactivity was more frequently found in the presynaptic side (82.4%) than in the postsynaptic side (17.6%; SEM = 1.8, Fig. 1d), and it was not observed in negative controls (blocking peptide, Supplementary Fig. S2). The presynaptic location of VPS35 was verified by immuno-gold electron microscopy using two different antibodies against VPS35 detected by Protein A-gold 10 nm. VPS35 immunosignal of both antibodies was detected inside presynaptic terminals (Fig. 1e-f, Supplementary Fig. S1), but not in the negative controls (absence of primary antibody, Supplementary Fig. S2)." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Confocal |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2625 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |