| Annotated protein: | Flotillin-2 (Epidermal surface antigen) (ESA) (Membrane component chromosome 17 surface marker 1 homolog). Gene symbol: FLOT2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q60634 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ FLOT2 |
| Ontology domain: | Biological Process |
| SynGO term: | anterograde dendritic transport (GO:0098937) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Bodrikov V, et al. "Reggie-1 and reggie-2 (flotillins) participate in Rab11a-dependent cargo trafficking, spine synapse formation and LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons" Exp Neurol. 2017 Mar;289:31-45 PMID:27993509 |
| Figure(s): | Fig. 1, Fig. 2, Fig. 3 |
| Annotation description: | Flotillin-2 regulates in cooperation with Rab11a membrane trafficking processes in spines and participates in LTP-related AMPA receptor (GluA1) surface exposure in mouse hippocampal neurons. 13/4/2017 Pim Fig.1 A-G - shRNA KD of Reggie1 or reggie2 results in less spines --> regulation of dendritic spine organization - Reggie1 KO mouse no effect on spine number, but less spine synapses --> regulation of synapse organization Fig.1 G-J - Reggie1 and Reggie2 localize to synaptic component transport vesicles in dendrites. Fig.2 A,B - RFP-Reggie1 is co-transported with PSD-95 in dendrites Fig.2 C-Fig - Reggie1 KD causes reduction in number of spine synapses (measured as juxtaposed presynaptic FM4-64FX and postsynaptic PSD-95-EGFP staining). Fig.3 - Reggie1 KO cells show impaired transport of PSD-95, and reduced number of synapses (measured as juxtaposed synaptophysin/PSD-95 staining) |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | NMDA receptors interact with flotillin-1 and -2 @ PMID:19298817 |
| SynGO annotation ID: | 262 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |