| Annotated protein: | Disks large homolog 3 (Synapse-associated protein 102) (SAP-102) (SAP102). Gene symbol: DLG3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P70175 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DLG3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Elias GM, et al. "Synapse-specific and developmentally regulated targeting of AMPA receptors by a family of MAGUK scaffolding proteins" Neuron. 2006 Oct 19;52(2):307-20 PMID:17046693 |
| Figure(s): | Figures 5, 8 |
| Annotation description: | SAP-102 is the dominant PSD-MAGUK responsible for the synaptic targeting of AMPA receptors during early postnatal development. Previous work showed SAP-102 protein is expressed strongly by postnatal day 10 (P10, robust PSD-95 and PSD-93 expression occurs 3 weeks later, Sans et al., 2000). "SAP-102 is not required for synaptic AMPA or NMDA receptor expression at mature synapses." shRNA knockdown of endogenous SAP-102 in pyramidal neurons had no effect on basal synaptic transmission (Fig. 5C1- 5C3). "SAP-102 shRNA caused an ~50% reduction specifically in AMPA-R-mediated synaptic transmission in immature synapses. (Fig. 8G1-8G3)." Furthermore, shRNA knockdown of PSD-95 did not affect synaptic transmission in immature synapses (Fig. 8F1-8F3) supporting the notion that SAP102 is the dominant PSD-MAGUK at this age. Figure 1. shRNAs Against PSD-95, PSD-93, and SAP-102 Cause Selective Knockdown in COS-7 Cells and Hippocampal Neurons. Figure 9. "Slice Culture Scheme" shows how mature vs immature synaptic transmission was assayed in hippocampal slices. |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) RNAi / shRNA |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Electrophysiology (generic) |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Figure 3: "Patterns of developmental expression of MAGUKs. P2, P10, P35, and 6 month (6M) rat hippocampus homogenates." Western blot analysis. Figure 4-5: Immunogold labeling of SAP102 and PSD-95 in the CA1 stratum radiatum of the hippocampus during development. Table 2. "Summary of immunogold labeling for PSD-95 and SAP-102" @ PMID:10648730 |
| SynGO annotation ID: | 2594 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |