| Annotated protein: | Glutamate receptor ionotropic, delta-2 (GluD2) (GluR delta-2 subunit). Gene symbol: GRID2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61625 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRID2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | Parallel fibre (cerebellum -> Purkinje-cell) |
| Annotated paper: | Matsuda K, et al. "Cbln1 is a ligand for an orphan glutamate receptor delta2, a bidirectional synapse organizer" Science. 2010 Apr 16;328(5976):363-8 PMID:20395510 |
| Figure(s): | Supplemental Figure S11 |
| Annotation description: | GluD2 is localizes to the postsynaptic density membrane of Parallel Fiber (PF)-Purkinje cells synapses in the cerebellum. Supplementary Fig. S11: Immunogold labeling for GluD2 by SDS-digested freeze-fracture replica labeling in the molecular layer of cerebellar tissue from WT and GluD2-KO mice. "Immunoparticles for GluD2 (10 nm) accumulated over a postsynaptic membrane specialization...in wild-type (A), but not in GluD2-null (B), mouse cerebellum." The number of GluD2 particles at these synapses was positively correlated with the synapse size (E). Double labeling of AMPARs (anti-pan GluA, 5 nm particles) and GluD2 (15 nm particles) in a Sprague-Dawley rat showed a similar distribution of both proteins at PF-PC synapses. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Genetic transformation (eg; knockout, knockin, mutations) Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| SynGO annotation ID: | 2593 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |