| Annotated protein: | MAGUK p55 subfamily member 2 (Discs large homolog 2) (Protein MPP2). Gene symbol: MPP2. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q9WV34 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MPP2 |
| Ontology domain: | Cellular Component |
| SynGO term: | anchored component of postsynaptic density membrane (GO:0099031) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Kim G, et al. "Membrane palmitoylated protein 2 is a synaptic scaffold protein required for synaptic SK2-containing channel function" Elife. 2016 Feb 12;5:e12637 PMID:26880549 |
| Figure(s): | Fig. 3 |
| Annotation description: | MPP2 is a synaptic MAGUK protein that localizes to the postsynaptic density of dendritic spines on CA1 pyramidal neurons. Fig. 3C-D. Pre-embedding immuno-gold electron microscopy (iEM) analysis in the stratum radiatum of the CA1 region of the hippocampus showed MPP2 localizes primarily to dendritic spines (84%) versus in dendrites. The majority of MPP2 immunoparticles detected in either compartment were found along the plasma membrane (84% of MPP2 in spines and 65% of MPP2 in dendrites). Post-embedding iEM showed showed significant labeling of MPP2 in the post-synaptic densities of dendritic spines. MPP2 was not detected in stratum pyramidale or in presynaptic terminals. Fig. 5. Validation of MPP2-shRNA knockdown and specificity of MPP2 antibody using pre-embedding iEM was performed on hippocampal sections from MPP2 shRNA-transfected animals. MPP2 particles in spines and dendrites were significantly reduced in MPP2-knockdown neurons compared control, untransfected cells (81% and 78%, respectively). - Based on the predicted lipid anchor of the protein, the "anchored component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Hana Goldschmidt (ORCID:0000-0002-5676-366X) Richard Huganir (ORCID:0000-0001-9783-5183) |
| Lab: | Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA and Kavli Neuroscience Discovery Institute, Johns Hopkins University, Baltimore, MD 21205, USA |
| Additional literature: | Fig 1. "MPP2 is a component of postsynaptic receptor complexes." Immunoprecipitation of TARP γ-2 from crude synaptosomal fractions showed MPP2 is present in AMPAR complexes containing GluA2, PSD-95 and TARP γ-2 (Fig.2A). MPP2 localizes to excitatory synapses (Fig. 1B). Immunostaining of cultured hippocampal neurons showed endogenous MPP2 is exclusively found in neurons (MAP2 positive cells) and MPP2 puncta along dendrites colocalized with with PSD-95 and vGlut1. Fig. 2. MPP2 interacts with the postsynaptic density proteins PSD-95 and GKAP. The interaction between MPP2 with PSD-95 was demonstrated immunoprecipitation of endogenous MPP2 from crude synaptosomes isolated from adult rat brain tissue (Fig. 2B). In heterologous cells, MPP2 was capable of binding PSD-95 independent of its PDZ and L27 domains (Fig. 2A, 2C). @ PMID:27756895 |
| SynGO annotation ID: | 2584 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |