| Annotated protein: | Ephrin type-A receptor 4 (EC 2.7.10.1) (Tyrosine-protein kinase receptor MPK-3) (Tyrosine-protein kinase receptor SEK-1). Gene symbol: EPHA4. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q03137 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPHA4 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus, glutamatergic neocortex, glutamatergic thalamus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Bouvier D, et al. "Pre-synaptic and post-synaptic localization of EphA4 and EphB2 in adult mouse forebrain" J Neurochem. 2008 Jul;106(2):682-95 PMID:18410519 |
| Figure(s): | fig 3, 4, 5, 6, 7, and 8. table 1 |
| Annotation description: | Areas of interest: latero-ventral hippocampus (CA1 radiatum and CA3 lucidum-radiatum), the deep layers (V-VI) of the primary motor cortex Using electron micrographs, EphA4 was detected with anti-EphA4 immunoperoxidase labeling in the adult mouse hippocampus within axon terminals and dendritic spines. Some dendritic shafts were occasionally labeled in the mouse hippocampus. No immunoperoxidase staining was found in EphA4 KO mice. Using pre-embedding immunogold labeling in electron micrographs of CA1, anti-EphA4 silver-intensified immunogold particles (SIGPs) were significantly concentrated in axon terminals (plasma membrane and vesicles). Fig 3. On Western Blots, EphA4 is detected in presynaptic active zone fractions from purified synaptosomes that are positive for SNAP25 and synaptophysin (and negative for PSD95 and GluR2/3) Fig 4. EphA4 is preferentially detected in axon terminals and dendritic spines in area CA3 of hippocampus, using immunoperoxidase staining in EM. Fig 5 and quantification in Fig 6. EphA4 is preferentially detected in axon terminals in area CA1 of hippocampus, using pre-embedding immunogold labeling in EM. Fig 7 and quantification in Fig 8. At the organelle level using pre-embedding immunogold labeling in EM, EphA4 is detected: at vesicles, plasma membrane, and other (core of the profiles but not clearly attributable to specific organelles) of axon terminals in area CA1 of hippocampus; majority at vesicles, but also at plasma membrane and other in CA3 and motor cortex. 2017/6/2017 Pim - "integral component .." inferred from transmembrane topology of EphB2. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 258 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |