| Annotated protein: | Ephrin type-B receptor 2 (EC 2.7.10.1) (Neural kinase) (Nuk receptor tyrosine kinase) (Tyrosine-protein kinase receptor EPH-3) (Tyrosine-protein kinase receptor SEK-3) [Cleaved into: EphB2/CTF1; EphB2/CTF2]. Gene symbol: EPHB2. Taxonomy: Mus musculus (Mouse). Uniprot ID: P54763 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ EPHB2 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic membrane (GO:0099056) |
| Synapse type(s): | hippocampus, glutamatergic thalamus, glutamatergic |
| Annotated paper: | Bouvier D, et al. "Pre-synaptic and post-synaptic localization of EphA4 and EphB2 in adult mouse forebrain" J Neurochem. 2008 Jul;106(2):682-95 PMID:18410519 |
| Figure(s): | Figs. 3, 4, 5, 6, 7, and 8. table 1 |
| Annotation description: | Areas of interest: latero-ventral hippocampus (CA1 radiatum and CA3 lucidum-radiatum), the deep layers (V-VI) of the primary motor cortex. Electron Micrographs of sectioned mouse brains were analyzed and quantified for immunoperoxidase labeling and silver-intensified immunogold particles detecting EphB2 using a anti-EphB2 antibody that recognizes mouse and human EphB2, but not EphB1, EphB3, or EphB4. In the ventrobasal thalamus, EphB2-associated particles (SIGPs) were more frequently found in axon terminals and also in small dendritic shafts. EphB2-associated particles (SIGPs) were concentrated on the plasma membrane of axon terminals in CA1. EphB2 is associated with plasma membrane of axon terminals in CA1. Fig 3. On Western Blots, EphB2 is detected in presynaptic active zone fractions from purified synaptosomes that are positive for SNAP25 and synaptophysin (and negative for PSD95 and GluR2/3) Fig 4. EphB2 rarely can be found in axon terminals in area CA3 of hippocampus, using immunoperoxidase staining in EM. Fig 5 and quantification in Fig 6. EphB2 is detected in predominantly in axon terminals thalamus and in both axon terminals and spines in hippocampus and cortex, using pre-embedding immunogold labeling in EM. Fig 7 and quantification in Fig 8. At the organelle level using pre-embedding immunogold labeling in EM, EphB2 is detected: at vesicles, plasma membrane, and other (core of the profiles but not clearly attributable to specific organelles) of axon terminals in thalamus; and at vesicles and plasma membrane and other in CA1, CA3, and motor cortex. 2017/6/2017 Pim - "integral component .." inferred from transmembrane topology of EphB2. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy Western blot Biochemical fractionation (generic) |
| Annotator(s): | Tony Cijsouw (ORCID:0000-0003-0912-1514) Thomas Biederer (ORCID:0000-0002-3670-7863) |
| Lab: | Department of Neuroscience, Tufts University School of Medicine, Boston, MA 02111, USA |
| SynGO annotation ID: | 257 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |