| Annotated protein: | Growth hormone secretagogue receptor type 1 (GHS-R) (GH-releasing peptide receptor) (GHRP) (Ghrelin receptor). Gene symbol: GHSR. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O08725 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GHSR |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of synaptic membrane (GO:0099699) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Ribeiro LF, et al. "Ghrelin triggers the synaptic incorporation of AMPA receptors in the hippocampus" Proc Natl Acad Sci U S A. 2014 Jan 7;111(1):E149-58 PMID:24367106 |
| Figure(s): | Fig.1 |
| Annotation description: | Ghrelin is a peptide that acts on the GHS-R1a receptor in hippocampal neurons to induce synaptic potentiation. Fig.1: "GHS-R1a localizes to excitatory synapses and is developmentally regulated in the hippocampus." - "To evaluate the presence of GHS-R1a at excitatory synapses, we identified regions of overlap between the PSD-95 and Vglut1 signals and measured the GHS-R1a immunolabeling at these sites (Fig. 1B). We found that 31 ± 0.02% of the clusters positive for both PSD-95 and Vglut1, presumably corresponding to functional synapses, contain GHS-R1a (Fig. 1 B and C), which accounts for 36 ± 7.3% of the total GHS-R1a fluorescence intensity in dendrites. Interestingly, only 23 ± 0.01% of the PSD-95 positive sites contain GHS-R1a (Fig. 1C), suggesting that GHS-R1a is preferentially localized to the vicinity of functional synapses. This distribution pattern was confirmed in primary hippocampal neurons transfected with GFP-tagged GHS-R1a (30) and immunolabeled at 16 DIV for GFP (Fig. S1 A-C). Furthermore, we observed that GHS-R1a is enriched in crude synaptosomes (P2 fraction) purified from adult rat hippocampi (Fig. 1D), consistent with a synaptic expression of GHS-R1a in the adult rat hippocampus." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Over-expression Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2569 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |