| Annotated protein: | Muscarinic acetylcholine receptor M3. Gene symbol: CHRM3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P08483 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CHRM3 |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of postsynaptic density membrane (GO:0099061) |
| Synapse type(s): | striatum, glutamatergic |
| Annotated paper: | Hersch SM, et al. "Distribution of m1-m4 muscarinic receptor proteins in the rat striatum: light and electron microscopic immunocytochemistry using subtype-specific antibodies" J Neurosci. 1994 May;14(5 Pt 2):3351-63 PMID:8182478 |
| Figure(s): | Fig.6 |
| Annotation description: | Fig.6: "Electron micrographs demonstrating the subcellular localization of m3 receptor protein" - "C, m3-immunoreactive dendrite (d) with minimal dendritic label but much more in a spine head (arrow) arising from it. D and E, m3-positive dendritic spines (s) forming asymmetrical synapses with unlabeled axon terminals (a). The upper spine (D) demonstrates intense labeling of the postsynaptic density (arrow). The lower spine (E) contains label that is primarily adjacent to the postsynaptic density (arrow). F and G, m3-immunoreactive myelinated axon (a) and unmyelinated preterminal axon (arrow). Adjacent, unlabeled preterminal axons are evident for comparison (triangles). H and I, m3-positive axon terminals (a) forming asymmetrical synapses with unlabeled dendritic spines (s). In contrast to the other muscarinic subtypes, reaction product (arrows) is distant from the active zones of the synapses." - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2557 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |