Annotated protein:	Muscarinic acetylcholine receptor M2. Gene symbol: CHRM2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P10980
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SynGO gene info:	SynGO data @ CHRM2
Ontology domain:	Cellular Component
SynGO term:	integral component of presynaptic membrane (GO:0099056)
Synapse type(s):	striatum, glutamatergic
Annotated paper:	Hersch SM, et al. "Distribution of m1-m4 muscarinic receptor proteins in the rat striatum: light and electron microscopic immunocytochemistry using subtype-specific antibodies" J Neurosci. 1994 May;14(5 Pt 2):3351-63 PMID:8182478
Figure(s):	Fig.5
Annotation description:	Fig.5: "Electron micrographs demonstrating the subcellular localization of m2 receptor protein" - " Electron micrograph of a rare somatic synapse between an unlabeled axon terminal (a) and an m2-immunoreactive neuron (~2). Reaction product is not present postsynaptically but is plentiful outside the region of this synapse (arrows)." - "D, Junctional complex (arrow) binding two m2-immunoreactive dendrites. The junctional densities appear intensely immunoreactive." - "F, m2-positive axon terminal (a) and preterminal axon (arrow). Synaptic vesicles appear to be surrounded by reaction product." - A junctional complex is not a synapse. - Based on the known transmembrane topology of the protein, the "integral component of ... membrane" term was chosen.
Evidence tracking, Biological System:	Intact tissue
Evidence tracking, Protein Targeting:	Antibody (detection)
Evidence tracking, Experiment Assay:	Electron Microscopy
Annotator(s):	Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216)
Lab:	Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands
SynGO annotation ID:	2556
Dataset release (version):	20231201
View annotation as GO-CAM model: