| Annotated protein: | D(1B) dopamine receptor (D(5) dopamine receptor) (Dopamine D5 receptor). Gene symbol: DRD5. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P25115 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DRD5 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | ventral tegmental area, glutamatergic |
| Annotated paper: | Schilstrom B, et al. "Cocaine enhances NMDA receptor-mediated currents in ventral tegmental area cells via dopamine D5 receptor-dependent redistribution of NMDA receptors" J Neurosci. 2006 Aug 16;26(33):8549-58 PMID:16914681 |
| Figure(s): | Fig.2, 5 |
| Annotation description: | Fig.2: "Cocaine enhances NMDAR EPSCs via activation of DA D1/D5 receptors" - "Together, these results suggest that DA D5 receptor activation during exposure to cocaine leads to a slowly developing enhancement of NMDAR EPSCs and that simultaneous activation of presynaptic DA D2 receptors can delay this effect, likely by reducing glutamate release." Fig.5: "Acute exposure to cocaine induces an increase in NR1 and NR2B subunit immunoreactivity in the VTA" - "A decrease of NR1 and NR2B was found in the light membrane fraction (P3) (NR1, 41 ± 3%; NR2B, 39% ± 11; n = 3; p < 0.05 relative to saline), containing cellular organelles such as endoplasmic reticulum and Golgi, and no changes in the levels of NR2A or NR2C were observed (Fig. 6A)" Note: SKF81297 is a non-selective D1/D5 agonist. SCH23390 is a non-selective D1/D5 antagonist. The authors note: "Presently, there are no pharmacological agents available to distinguish D1 receptors from D5 receptors. However, neither D1 receptor mRNA nor protein has been found in DA cell bodies (Weiner et al., 1991; Yung et al., 1995); therefore, any action of mixed D1/D5 agents in midbrain DA cells is likely mediated via DA D5 receptors. Figure 2B shows that bath application of the DA D1/D5 receptor agonist SKF81297 (10 μm; 10 min) enhanced NMDAR EPSCs (132 ± 5%; n = 8; p < 0.01). Moreover, pretreatment of slices with the DA D1/D5 receptor antagonist SCH23390 (10 μm) prevented the potentiation of NMDAR EPSCs by cocaine (97 ± 12%; n = 4; p > 0.05) (Fig. 2C), suggesting that stimulation of D5 receptors is both necessary and sufficient for cocaine to induce an increase of NMDAR EPSCs." So, the selective expression of D5 is fundamental in deciding the gene specific effect. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Western blot Biochemical fractionation (generic) |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2542 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |