| Annotated protein: | Neurogranin (Ng) (Protein kinase C substrate 7.5 kDa protein) (RC3) [Cleaved into: NEUG(55-78)]. Gene symbol: NRGN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q04940 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NRGN |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | striatum, glutamatergic |
| Annotated paper: | Watson JB, et al. "Localization of the protein kinase C phosphorylation/calmodulin-binding substrate RC3 in dendritic spines of neostriatal neurons" Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8581-5 PMID:1528865 |
| Figure(s): | Fig. 4c,d |
| Annotation description: | Figure 4 Legend: -Literal: "Within puncta of the neuropil, RC3-1 and RC3-5 antisera revealed immunolabeling mainly in dendritic spines (S) forming asymmetrical synaptic junctions with axonal terminals (AT) (C and D)." Results section: "Ultrastructure of RC3 Neurons." -Literal: "In puncta, RC3 [neurogranin, nrgn] immunolabeling was observed principally in dendritic shafts and dendritic spines. Dendritic shafts and spine necks contained slight traces of RC3. In contrast, RC3-containing spine heads were the most frequent immunolabeled profiles and had the heaviest deposits of label in all cases (Fig. 4 C and D). |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2540 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |