| Annotated protein: | 14-3-3 protein epsilon (14-3-3E) (Mitochondrial import stimulation factor L subunit) (MSF L). Gene symbol: YWHAE. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62260 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ YWHAE |
| Ontology domain: | Cellular Component |
| SynGO term: | synapse (GO:0045202) |
| Synapse type(s): | brain |
| Annotated paper: | Martin H, et al. "Subcellular localisation of 14-3-3 isoforms in rat brain using specific antibodies" J Neurochem. 1994 Dec;63(6):2259-65 PMID:7964746 |
| Figure(s): | fig 3, fig 4 |
| Annotation description: | From Results section: The 1.0/1.3 M sucrose interface consists primarily of synaptic plasma membranes with a portion of their postsynaptic membrane and postsynaptic density attached. (...) Using the anti-14-3-3 antiserum as a probe, the immunoreactivity was most concentrated in the M1.0 and synaptic plasma membrane fractions. To characterise further the location of the 14-3-3 in the synaptic membrane preparation, the iodonitrotetrazolium-treated membranes were extracted with 0.2% Triton X-100, and the Triton-soluble and -insoluble components were separated by centrifugation through a layer of 1 M sucrose. (...) The ß/ζ isoforms appeared to be quantitatively solubilised by the treatment, whereas the isoforms γ, ε, and η were present in each of the three fractions at similar concentrations . This suggests that some of the γ, ε, and η isoforms may be associated with postsynaptic densities. To investigate further the association of 14-3-3 isoforms with synapses, we isolated synaptosomes using the Percoll gradient procedure. (...) interface 3 contains many small but often slightly damaged synaptosomes as well as plasma membranes ; interface 4 is highly enriched in intact viable synaptosomes, most of which have attached postsynaptic membranes with postsynaptic densities; (...) The ß and ζ isoforms were not detectable in the membranes in fraction 4, whereas the γ and ε isoforms were present. |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Western blot Biochemical fractionation (generic) |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2536 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |