| Annotated protein: | D(3) dopamine receptor (Dopamine D3 receptor). Gene symbol: DRD3. Taxonomy: Mus musculus (Mouse). Uniprot ID: P30728 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DRD3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of neurotransmitter uptake (GO:0051580) |
| Synapse type(s): | striatum, dopaminergic |
| Annotated paper: | McGinnis MM, et al. "Dopamine D3 autoreceptor inhibition enhances cocaine potency at the dopamine transporter" J Neurochem. 2016 Sep;138(6):821-9 PMID:27393374 |
| Figure(s): | Fig.1, 3 |
| Annotation description: | Activation of D2/D3 autoreceptors does not regulate dopamine release, but modulates the inhibitory effect of cocaine on dopamine reuptake. Fig.1: "Global autoreceptor blockade enhances cocaine's ability to inhibit dopamine uptake at the dopamine transporter" - "Following bath application of cocaine, we found that cocaine-induced increases in apparent Km for DA uptake were greater in the presence of raclopride than in control slices which were run under raclopride-free bath conditions (Figure 1C)" Fig.3: "D3, but not D2, autoreceptor blockade increases the ability of cocaine to inhibit dopamine uptake" - "Using the D2-receptor selective antagonist, L-741,626 (30 nM), and the D3-receptor selective antagonist, SB-277011-A (300 nM), we sought to determine whether the increase in cocaine potency that was observed in the presence of raclopride was mediated through DAT functional interactions with the D2 or D3 autoreceptor ... we found that cocaine-induced uptake inhibition was amplified in the presence of the D3-receptor selective antagonist, SB-277011-A (Figure 3E)" |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Voltammetry assay |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2526 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |