| Annotated protein: | D(3) dopamine receptor (Dopamine D3 receptor). Gene symbol: DRD3. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P19020 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DRD3 |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic neurotransmitter receptor endocytosis (GO:0099149) |
| Synapse type(s): | striatum, GABAergic |
| Annotated paper: | Chen G, et al. "Dopamine D3 receptors regulate GABAA receptor function through a phospho-dependent endocytosis mechanism in nucleus accumbens" J Neurosci. 2006 Mar 1;26(9):2513-21 PMID:16510729 |
| Figure(s): | Fig.1, 2, 4, 5, 7 |
| Annotation description: | Fig.1: "Activation of dopamine D3 receptors reduced GABAA receptor-mediated current in nucleus accumbens" - "As shown in Figure 1D-F, bath application of PD128907 (10 μm) caused a strong reduction of the amplitude and frequency of mIPSC, which were stable throughout the recording period when no agonist was applied." Fig.2: "The dynamin inhibitory peptide blocked the D3 effect on GABAA receptors" - "As shown in Figure 2, D and E, PD128907 was without effect on mIPSC amplitude in the cell dialyzed with the p4 peptide, whereas inclusion of a scrambled control peptide failed to block the PD128907 reduction of mIPSC amplitude." Fig.4: "PKA was specifically involved in the D3 regulation of GABAAR current" - Note: DRD3 is known to signal through PKA. Fig.5: "PKA phosphorylation-dependent endocytosis of GABAAR β subunit was involved in the D3 modulation of postsynaptic GABAA receptors" Fig.6: "Activation of NAc D3 receptors increased GABAAR internalization and reduced the GABAAR surface expression in a dynamin- and PKA-dependent manner." - Fig.7: "D3 receptor activation increases NAc neuron excitability through a mechanism involving GABAA receptors" |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Wide-field fluorescence Whole-cell patch clamp Western blot |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2524 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |