| Annotated protein: | D(1A) dopamine receptor (Dopamine D1 receptor). Gene symbol: DRD1. Taxonomy: Mus musculus (Mouse). Uniprot ID: Q61616 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ DRD1 |
| Ontology domain: | Biological Process |
| SynGO term: | presynaptic modulation of chemical synaptic transmission (GO:0099171) |
| Synapse type(s): | striatum |
| Annotated paper: | Wang W, et al. "Regulation of prefrontal excitatory neurotransmission by dopamine in the nucleus accumbens core" J Physiol. 2012 Aug 15;590(16):3743-69 PMID:22586226 |
| Figure(s): | Fig.1, 2, 6 |
| Annotation description: | Fig.1: "Amphetamine and endocannabinoids produce frequency-dependent filtering at corticoaccumbal terminals" Fig.2: "D1R-mediated inhibition of corticoaccumbal release requires adenosine" - "Since presynaptic filtering by amphetamine was generated at 20 Hz, we used this stimulation frequency to determine how corticoaccumbal inhibition was produced through D1Rs. Similar to amphetamine, the D1R agonist SKF38393 increased FM1-43 destaining (t1/2 = 270 s for a SKF38393, n = 193 puncta vs. t1/2 = 207 s for vehicle, n = 330; P < 0.001, Mann-Whitney U test) (Fig. 2A and B), by inhibiting terminals with the lowest probability of release (Supplementary Fig. S5)." Fig.6: "D1 and A1 adenosine receptors modulate presynaptic activity in fluorescent cells from Drd1-EGFP mice" - "To further localize D1 and A1 receptors on cortical afferents of fluorescent MSNs in Drd1-EGFP mice, miniature (m) EPSCs were observed in the presence of the Na+ channel antagonist TTX, which blocked spontaneous cortically derived action potentials and isolated activity at the presynaptic terminal. The D1R agonist SKF38393 increased the frequency of mEPSC by 35 ± 16% (4.77 ± 0.91 Hz in vehicle vs. 6.51 ± 1.34 Hz in SKF38393; n = 9; P = 0.04, Student's paired t test), by selectively increasing high-frequency, low-amplitude inward currents (Fig. 6A and B). There was no difference in the distribution of mEPSC amplitudes (Fig. 6B), indicating that the increase in mEPSCs frequency was likely to be attributable to the potentiating effect of SKF38393 on presynaptic release (Van der Kloot, 1991)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Whole-cell patch clamp Optical physiology |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2519 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |