| Annotated protein: | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP-1A) (EC 3.1.3.16). Gene symbol: PPP1CA. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P62138 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PPP1CA |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynapse (GO:0098794) |
| Synapse type(s): | caudateputamen, glutamatergic |
| Annotated paper: | Ouimet CC, et al. "The alpha and gamma 1 isoforms of protein phosphatase 1 are highly and specifically concentrated in dendritic spines" Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3396-400 PMID:7724573 |
| Figure(s): | Fig.3 |
| Annotation description: | Fig.3: "Electron and light photomicrographs of PP1 immunoreactivity in the caudatoputamen. (A and B) Electron micrographs of immunoreactivity for PPla in the caudatoputamen. (A) Robust immunoreactivity for PP1a is present in dendritic spine heads (S). Weak immunoreactivity is present in dendritic shafts (D). Some axon terminals are also immunoreactive (AT). (Inset) The postsynaptic density (PSD; arrowhead) in the dendrite (D) is labeled." - "At the electron microscopic level, dendritic spine heads and spine necks were strongly immunoreactive (Fig. 3 A and B). Immunoreactivity was also observed in PSDs, but this immunoreactivity must be interpreted with caution, since immunoreaction product generated elsewhere in the spine could diffuse to the PSD and become fixed to it during osmication." - Note: the authors do only show the data from PPP1CA, but state: "Similar images are observed for PPlgamma1 (not shown)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Electron Microscopy |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2518 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |