| Annotated protein: | Multiple PDZ domain protein (Multi-PDZ domain protein 1). Gene symbol: MPDZ. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O55164 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ MPDZ |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Krapivinsky G, et al. "SynGAP-MUPP1-CaMKII synaptic complexes regulate p38 MAP kinase activity and NMDA receptor-dependent synaptic AMPA receptor potentiation" Neuron. 2004 Aug 19;43(4):563-74 PMID:15312654 |
| Figure(s): | Fig. 1 |
| Annotation description: | Figure 1 Legend: Literal: "Figure 1. (A) MUPP1 is enriched in postsynaptic densities (PSDs). Protein (10 g) from rat brain P2 membrane, purified synaptosomes (Syn), and PSDs were probed by Western blot with Mpdz4 and PSD-95 antibodies. The amount of MUPP1 in PSDs increased in parallel with the PSD protein marker PSD-95. (B) MUPP1 colocalized with PSD-95 in synapses of rat hippocampal neurons (18 d.i.v.). Neurons were labeled with Mpdz4 (red) and PSD-95 (green). (C) Both MUPP1 and NR1 were predominantly in the pellet (P) of synaptosomal membranes extracted with Triton X-100 and almost completely solubilized (S) with alkaline sodium deoxycholate (DOC). (D) Two different MUPP1 antibodies coimmunoprecipitated the NMDAR subunit, NR1, and PSD-95 from solubilized synaptosomes. After probing with NR1 antibody, the blot was stripped and reprobed with PSD-95 antibody." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Rita Reig-Viader (ORCID:0000-0002-6893-6177) Àlex Bayés (ORCID:0000-0002-5265-6306) |
| Lab: | Molecular Physiology of the Synapse Laboratory, Biomedical Research Institute Sant Pau, 08025 Barcelona, Spain and and Universitat Autnoma de Cerdanyola del Valls, Spain Barcelona, 08193 Bellaterra |
| SynGO annotation ID: | 2503 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |