| Annotated protein: | Protein kinase C iota type (EC 2.7.11.13) (Atypical protein kinase C-lambda/iota) (aPKC-lambda/iota) (nPKC-iota). Gene symbol: PRKCI. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: F1M7Y5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PRKCI |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynaptic membrane neurotransmitter receptor levels (GO:0099072) |
| Synapse type(s): | hippocampus, glutamatergic Schaffer collateral synapse (CA3->CA1) |
| Annotated paper: | Ren SQ, et al. "PKClambda is critical in AMPA receptor phosphorylation and synaptic incorporation during LTP" EMBO J. 2013 May 15;32(10):1365-80 PMID:23511975 |
| Figure(s): | Figure 1-3 |
| Annotation description: | This publication provides some evidence that PKC lambda/iota regulates the insertion of AMPAR at the PSD. -A specific shRNA blocks the induction of LTP in hippocampal slices, which can be rescued by re-expression of PKC-lambda. (Figure 1) -PI3K activation increases PKC lambda auto-phosphorylation and EPSC amplitudes. This is inhibited by PKC-lambda shRNA, and an inhibitor (Myr-aPKC-PS, more commonly known as ZIP) with some specificity to PKC-lambda. (Figure 2). -PI3K activation leads to increased surface expression of GluRs, which is inhibited by Myr-aPKC-PS (Figure 3C-F). -PI3K activation leads to increased mEPSC amplitudes, which can be blocked by PKC-lambda shRNA and Myr-aPKC-PS (Figure 3G-H). It should be noted that at the concentration used, Myr-aPKC-PS also strongly inhibits PKC-zeta (see Figure 1D and Tsokas et al 2016). Thus, in several assays in this publication, it cannot be excluded that observed effects are mediated by PKC-zeta. The role of PKC-lambda in LTP is debated and likely involves an interplay with PKC-zeta expression (Tsokas et al 2016). |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Antagonist / agonist |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp Western blot |
| Annotator(s): | Arthur de Jong (ORCID:0000-0002-7620-2704) Pascal Kaeser (ORCID:0000-0002-1558-1958) |
| Lab: | Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA |
| Additional literature: | This publication provides additional discussion for the specificity of the inhibitors used. @ PMID:27187150 |
| SynGO annotation ID: | 250 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |