| Annotated protein: | Neuroplastin (Glycoprotein 55/65) (gp55/65) (Stromal cell-derived receptor 1) (SDR-1). Gene symbol: NPTN. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: P97546 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ NPTN |
| Ontology domain: | Cellular Component |
| SynGO term: | integral component of presynaptic active zone membrane (GO:0099059) |
| Synapse type(s): | brain, glutamatergic brain, GABAergic |
| Annotated paper: | Boyken J, et al. "Molecular profiling of synaptic vesicle docking sites reveals novel proteins but few differences between glutamatergic and GABAergic synapses" Neuron. 2013 Apr 24;78(2):285-97 PMID:23622064 |
| Figure(s): | fig. 5, supplementary tables S1 and S5 |
| Annotation description: | >5fold-enriched in docked SV fraction (fig. 5), 1.5fold enriched in glutamatergic synapses MS analysis after immunoisolation of synaptophysin (clone 7.2), VGLUT1 or VGAT positive synaptosomes 18/01/2017 Pim/Frank+Gundelfinger lab - Important is that NP is 6x enriched in purified active zone compared to purified SVs. This means that NP is most likely localized in the active zone membrane. Note that NP is also found in SV membrane (Takamori et al. paper PMID:17110340). - NP has TM domain |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Mass-spectrometry Biochemical fractionation (generic) |
| Annotator(s): | Daniela Dieterich (ORCID:0000-0002-9880-1214) Rainer Pielot (ORCID:0000-0002-9681-3318) Karl-Heinz Smalla (ORCID:0000-0002-0269-0311) Eckart Gundelfinger (ORCID:0000-0001-9377-7414) |
| Lab: | Leibniz Institute for Neurobiology (LIN), Institute of Pharmacology and Toxicology, Otto von Guericke University, Center for Behavioral Brain Sciences (CBBS), Magdeburg, Germany |
| Additional literature: | PMID:19187093 PMID:24534009 PMID:24554721 |
| SynGO annotation ID: | 25 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |