| Annotated protein: | Glutamate receptor. Gene symbol: GRIK1. Taxonomy: Macaca Mulatta (Rhesus Macaque). Uniprot ID: F7AGW3 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ GRIK1 |
| Ontology domain: | Biological Process |
| SynGO term: | transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential (GO:1904315) |
| Synapse type(s): | retina, glutamatergic |
| Annotated paper: | Puthussery T, et al. "Kainate receptors mediate synaptic input to transient and sustained OFF visual pathways in primate retina" J Neurosci. 2014 May 28;34(22):7611-21 PMID:24872565 |
| Figure(s): | Fig.3, 5, 6 |
| Annotation description: | Postsynaptic localizations of KARs are not frequently reported. This study shows that GRIK1; GluK1; GluR5 mediates glutamatergic postsynaptic responses in DB2 and DB3b cells. This study does not include perturbation of GRIK1 expression as part of the evidence but uses a combination of protein expression (DB2 and DB3b cells express GRIK1 whereas others do not) and the fast desensitization kinetics of GRIK1-containing KARs. Fig.3: "Cone to FMB EPSCs are mediated by KARs" - Rapidly desensitizing current of Cone->FMB synaptic transmission is bocked by KAR inhibitor ACET. Fig.5: "Diverse effects of the KAR agonist, ATPA, across OFF cone bipolar cell types. " - "To further examine these functional differences, we used the KAR agonist, ATPA (Hoo et al., 1999; DeVries, 2000; Paternain et al., 2000), which in heterologous expression systems produces desensitizing currents in GluK1/5 and GluK1/2 and nondesensitizing currents in GluK2/5 heteromers (Paternain et al., 2000). In the retina, we found that paired-puff application of 10 μm ATPA, with a 15 s interval, elicited sustained inward currents in FMB, DB1, and DB3a cells for both the first and second puff (Fig. 5A-C). By contrast, responses in DB2 and DB3b cells were relatively transient and remained strongly desensitized when the second puff was applied 15 s later (Fig. 5A-C)" - Note: the fast rate of desenstization is constent with GluK1-containing KARs Fig.6: "GluK1 expression in OFF-bipolar cells" - "In summary, our results suggest that DB2 and DB3b cells express GluK1 and have desensitizing responses to ATPA (Fig. 5)." |
| Evidence tracking, Biological System: | Intact tissue |
| Evidence tracking, Protein Targeting: | Antagonist / agonist Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Whole-cell patch clamp |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2496 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |