| Annotated protein: | Catenin delta-2. Gene symbol: CTNND2. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: O35116 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ CTNND2 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | hippocampus, glutamatergic |
| Annotated paper: | Silverman JB, et al. "Synaptic anchorage of AMPA receptors by cadherins through neural plakophilin-related arm protein AMPA receptor-binding protein complexes" J Neurosci. 2007 Aug 8;27(32):8505-16 PMID:17687028 |
| Figure(s): | Fig.4, 5 |
| Annotation description: | Fig.4: "Endogenous NPRAP is found at synapses of cultured hippocampal neurons and colocalizes with AMPA receptors" - "The great majority of spiny neurons expressed the postsynaptic synaptic marker PSD-95, and in a proportion of these neurons, NPRAP was also observed in spines, colocalizing with PSD-95 (Fig. 4A). The colocalization suggests that NPRAP is located at synapses along dendrites, in agreement with a dendritic localization for NPRAP in adult mouse brain (Ho et al., 2000), and implies a postsynaptic role for NPRAP. " - "In spiny neurons, NPRAP overlapped with GRIP puncta in spines and along the dendritic membrane (Fig. 4B), consistent with NPRAP interaction with GRIP (Fig. 5B,D; see below). In spiny neurons, NPRAP also colocalized with GluR2 in spines (Fig. 4C), consistent with its interaction with GRIP-GluR2 complexes (Fig. 1F). We conclude that NPRAP colocalizes with a synaptic marker, PSD-95, and with AMPARs, and with a subset of GRIP that is synaptic." - "We also observed NPRAP in a large proportion of aspiny neurons, in which NPRAP was less punctate and was found along the boundary of the dendrite, at which it appeared to line the plasma membrane. In these neurons, NPRAP colocalized frequently with GluR2 (Fig. 4D). Thus, NPRAP appeared in two different patterns of localization, one in spines, in which it was punctate, and the second in aspiny neurons, in which it appeared along the perimeter of the dendrite in a more continuous distribution. In both instances, it colocalized with GluR2." Fig.5: "PSD enrichment and interaction of endogenous NPRAP with N-cadherin and the scaffolding proteins ABP, GRIP, and PSD-95 in brain and synaptosomal fractions" - " we coimmunoprecipitated endogenous proteins from brain lysates. NPRAP coimmunoprecipitated with N-cadherin (Fig. 5A) and with GRIP, ABP, and PSD-95, but not with synaptophysin or with a control IgG immunoprecipitation (Fig. 5Bi,ii). This confirmed that NPRAP interacts with cadherins, ABP, GRIP, and PSD-95 in primary neurons. To assess the synaptic localization of these proteins, we assayed their presence in whole-brain Triton X-100 extracts and in synaptically enriched brain fractions (Fig. 5C). NPRAP, N-cadherin, GRIP, GluR2, and synaptophysin were all enriched in synaptosomes and, with the exception of the synaptic vesicle marker, synaptophysin, were greatly enriched in the PSD fraction (Fig. 5C). " FIg.6: " |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal Western blot Biochemical fractionation (generic) IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2485 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |