| Annotated protein: | Rap guanine nucleotide exchange factor 4 (Exchange factor directly activated by cAMP 2) (Exchange protein directly activated by cAMP 2) (EPAC 2) (cAMP-regulated guanine nucleotide exchange factor II) (cAMP-GEFII). Gene symbol: RAPGEF4. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: Q9Z1C7 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ RAPGEF4 |
| Ontology domain: | Cellular Component |
| SynGO term: | postsynaptic density (GO:0014069) |
| Synapse type(s): | cerebral cortex, glutamatergic |
| Annotated paper: | Woolfrey KM, et al. "Epac2 induces synapse remodeling and depression and its disease-associated forms alter spines" Nat Neurosci. 2009 Oct;12(10):1275-84 PMID:19734897 |
| Figure(s): | Fig.1, Sup.Fig.1, Sup.Fig.2 |
| Annotation description: | Fig.1: "Epac2 participates in postsynaptic protein complexes" - "Proteomic studies detected Epac2 in forebrain postsynaptic densities8,9. To investigate its synaptic localization we immunostained cultured cortical neurons with an antibody that detects a single protein band of ~110 kDa in rat cerebral cortex (Fig. 1c). In pyramidal neurons (div 28) we detected Epac2 in punctate structures along dendrites (Fig. 1d), and in the soma, suggesting a functional role in dendrites. Epac2 colocalized with the synaptic markers bassoon, GluR2/3, PSD-95, and NR1, indicating enrichment in excitatory synapses (Fig. 1d-e). Colocalization of Epac2, PSD-95, NR1, and GluR2/3 signals in dendrites is quantified in Supplementary Fig. 1a-b. Together with Supplementary Fig. 2a-e, these data show that a significant amount of Epac2 is present in synapses and spines, in addition to other subcellular compartments. Small puncta of Epac2 signal were also detected in axons and colocalized with tau (Supplementary Fig. 2g). Epac2 may thus participate in protein complexes with postsynaptic proteins. Epac2 coimmunoprecipitated with the postsynaptic density (PSD) scaffolding protein PSD-95 from rat forebrain homogenates (Supplementary Fig. 1d), indicating that they participate in the same postsynaptic protein complexes." |
| Evidence tracking, Biological System: | Intact tissue Cultured neurons |
| Evidence tracking, Protein Targeting: | Antibody (detection) |
| Evidence tracking, Experiment Assay: | Confocal IP + WB/MSMS |
| Annotator(s): | Pim van Nierop (ORCID:0000-0003-0593-3443) Guus Smit (ORCID:0000-0002-2286-1587) Matthijs Verhage (ORCID:0000-0002-2514-0216) |
| Lab: | Department of Functional Genomics, Department of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Vrije Universiteit Amsterdam, 1081 HV Amsterdam, The Netherlands |
| SynGO annotation ID: | 2482 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |