| Annotated protein: | Pleckstrin and Sec7 domain containing. Gene symbol: PSD. Taxonomy: Rattus norvegicus (Rat). Uniprot ID: G3V8J5 |
| antibody wiki: | |
| SynGO gene info: | SynGO data @ PSD |
| Ontology domain: | Biological Process |
| SynGO term: | regulation of postsynapse assembly (GO:0150052) |
| Synapse type(s): | hippocampus |
| Annotated paper: | Choi S, et al. "ARF6 and EFA6A regulate the development and maintenance of dendritic spines" J Neurosci. 2006 May 3;26(18):4811-9 PMID:16672654 |
| Figure(s): | fig 3, 4, 5 |
| Annotation description: | EFA6A promotes spine formation through ARF6 activation in cultured hippocampal neurons. Knockdown of EFA6A and ARF 21/2/2018 Pim Fig.3: "EFA6A promotes spine formation through ARF6 activation" - " Consistently, EFA6A E242K caused an increase in the length of dendritic protrusions and decreases in protrusion width and PSD-95 localization in spines (Fig. 3E-J). These results suggest that EFA6A promotes spine formation in an ARF6-dependent manner."6 in cultured hippocampal neurons by siRNA results in a decrease in the density of dendritic spines. Fig.4: "The C-terminal (PH+C) region of EFA6A leads to an increase in spine density" - This is caused by an increase in formation of filopodia and is shown to be independent of ARF6. Fig.5: "Knockdown of EFA6A and ARF6 by siRNA results in a decrease in spine formation" - Knockdown of ARF6 in cultured neurons (DIV 13-16) led to a decrease in spine density, with a concomitant increase in filopodia density (Fig. 5A-D), probably because of a reduced filopodia-to-spine conversion...Knockdown of EFA6A also resulted in similar changes, although to a lesser extent in some parameters, compared with ARF6 knockdown (Fig. 5A-J)." Note: since EFA6A is believed to act via ARF6 via actin cytoskeleton (Rac1 mediated) I think the regulation is from going from a filopodium to a spine. I do not consider the filopodium a synapse. Hence, I categorized the role of EFA6A in synapse assembly rather synapse maturation. |
| Evidence tracking, Biological System: | Cultured neurons |
| Evidence tracking, Protein Targeting: | RNAi / shRNA Over-expression |
| Evidence tracking, Experiment Assay: | Confocal |
| Annotator(s): | Hwajin Jung (ORCID:0000-0001-6194-9648) Eunjoon Kim (ORCID:0000-0001-5518-6584) |
| Lab: | Center for Synaptic Brain Dysfunctions, Institute for Basic Science (IBS) and Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, South Korea |
| SynGO annotation ID: | 2460 |
| Dataset release (version): | 20231201 |
| View annotation as GO-CAM model: |  |